A new method for the preparation of liposomes is described that avoids the use of pharmaceutically unacceptable solvents and energy-expensive procedures such as Sonication. The method is based on the initial formation of a pro-liposome mixture containing lipid, ethanol and water, which is converted to liposomes by a simple dilution step. Measurements using 6-carboxyfluorescein as a marker indicate that water-soluble drugs can be trapped with extremely high efficiency (65–80% depending on lipid composition).
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